- A protocol for high quality DNA extraction for genome sequencing was provided by Francis Martin. This is a modified protocol from Prof Pietro Spanu and [Fulton et al]
- PDF for Genomic DNA Extraction Annegret Kohler, Francis Martin, et al.
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High quality genomic DNA extraction using PowerMax Soil DNA Isolation Kit (MoBio 12988-10) – modified by INRA group and detailed in this PDF: MObio_Powersoil_genomicDNAProtocol.
- A CTAB extract protocol from Sara Branco (UC Berkeley) used to extract DNA from Suillus brevipes and Wilcoxina mikolei in this PDF Short CTAB Extraction Protocol.
- T. M. Fulton, J. Chunwongse, S. D. Tanksley, Pl Mol Biol Rep 13, 207 (1995) DOI: 10.1007/BF02670897
Jason Stajich on Google+
The protocol use 0.1 mg of Proteinase K, that is per ml or per sample (17.5 ml of buffer)? In the calculation for the microprep buffer you use 125 ul of Proteinase K, so that doesn’t make any sense to me…could you explain? Please
Sorry for any confusion, I have uploaded a new version of the protocol from the INRA group which corrects the protocol.
kindly send me the protocol … i cant find the link here
The link is in the text on this page – http://1000.fungalgenomes.org/home/wp-content/uploads/2013/02/genomicDNAProtocol-AK0511.pdf
125 µl of of Proteinase K (20 mg/ml) translates into 2.5 mg. Could you please clarify it translates into how may units ? I am using Invitrogen where conc is about 20 units/mg. Accordingly, in this protocol 125 µl of of Proteinase K (20 mg/ml) translates into 50 units. Can I consider 50 units/500 mg of fungal tissue as a rule of thumb ?
Thanks,
Prasun Ray