Extraction of high quality DNA for Genome Sequencing

  • A protocol for high quality DNA extraction for genome sequencing was provided by Francis Martin. This is a modified protocol from Prof Pietro Spanu and [Fulton et al]
  • A CTAB extract protocol from Sara Branco (UC Berkeley) used to extract DNA from Suillus brevipes and Wilcoxina mikolei in this PDF Short CTAB Extraction Protocol.
  1. T. M. Fulton, J. Chunwongse, S. D. Tanksley, Pl Mol Biol Rep 13, 207 (1995) DOI: 10.1007/BF02670897

5 thoughts on “Extraction of high quality DNA for Genome Sequencing”

  1. The protocol use 0.1 mg of Proteinase K, that is per ml or per sample (17.5 ml of buffer)? In the calculation for the microprep buffer you use 125 ul of Proteinase K, so that doesn’t make any sense to me…could you explain? Please

  2. 125 µl of of Proteinase K (20 mg/ml) translates into 2.5 mg. Could you please clarify it translates into how may units ? I am using Invitrogen where conc is about 20 units/mg. Accordingly, in this protocol 125 µl of of Proteinase K (20 mg/ml) translates into 50 units. Can I consider 50 units/500 mg of fungal tissue as a rule of thumb ?
    Thanks,
    Prasun Ray

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