Pseudovirgaria hyperparasitica genome released

1KFG is pleased to announce the public release of the genome of Pseudovirgaria hyperparasitica CBS 121739  P. hyperparasitica  is part of an unclassified group of Dothideomycetes  (Ascomycota; Dothideomycetes) and its phylogenetic position will be evaluated as part of phylogenomic analyses in the 1KFG project.

Pseudovirgaria hyperparasitica  was contributed to the 1KFG project by Pedro Crous and Manfred Binder at CBS-KNAW.  As always, please contact the PIs associated with unpublished 1KFG genomes for permission prior to the use of any data in publications.

Tothia fuscella genome released

1KFG is pleased to announce the public release of the genome of Tothia fuscella CBS 130266  T. fuscella is classified in the family Microthyriaceae (Ascomycota; Dothideomycetes;Microthyriales), and is currently the first completed genome for the family and one of two targeted – the other is Microthyrium microscopicum which is nearing completion at JGI as well.

Tothia fuscella  was contributed to the 1KFG project by Pedro Crous and Manfred Binder at CBS-KNAW.  As always, please contact the PIs associated with unpublished 1KFG genomes for permission prior to the use of any data in publications.

Clathrospora elynae genome released

1KFG is pleased to announce the public release of the genome of Clathrospora elynae CBS 161.51.  C. elynae  is classified in the family Diademaceae (Ascomycota; Dothideomycetes;Pleosporales), and is currently one of two targeted completed genomes from this family.

Clathrospora elynae was contributed to the 1KFG project by Pedro Crous and Manfred Binder at CBS-KNAW.  As always, please contact the PIs associated with unpublished 1KFG genomes for permission prior to the use of any data in publications.

Verruculina enalia genome released

1KFG is pleased to announce the public release of the genome of Verruculina enalia  CBS 304.66V. enalia  is classified in the family Didymosphaeriaceae (Ascomycota; Dothideomycetes;Pleosporales), and is currently the only completed genome from this family and one of two targeted for the family.

Verruculina enalia  was contributed to the 1KFG project by Pedro Crous and Manfred Binder at CBS-KNAW.  As always, please contact the PIs associated with unpublished 1KFG genomes for permission prior to the use of any data in publications.

Verruculina enalia Image Credit: Pedro Crous

 

Polyplosphaeria fusca genome released

1KFG is pleased to announce the public release of the genome of Polyplosphaeria fusca  CBS 125425.  P. fusca  is classified in the family Tetraplosphaeriaceae (Ascomycota; Dothideomycetes;Pleosporales), and is currently the only completed genome from this family and one of two targeted for the family.

Polyplosphaeria fusca  was contributed to the 1KFG project by Pedro Crous and Manfred Binder at CBS-KNAW.  As always, please contact the PIs associated with unpublished 1KFG genomes for permission prior to the use of any data in publications.

Polyplosphaeria fusca – image from Pedro Crous

Protocols for fruiting body sampling

Recently 1KFG has begun exploring using fresh fruiting body sampled tissue to obtain DNA rather than requiring pure culture-based samples only. This can be problematic for a variety of reasons including the heterokaryon nature of fruiting tissue, possible contamination with bacteria, insects, or other fungi, and the limitations to return to the exact sample for additional sampling since it will either be destroyed in the sampling or only have a small amount left for a voucher specimen.

All of that noted, we still can only obtain samples of some fungi as fruiting mushrooms. The INRA-Nancy group have applied this approach of sampling fruiting tissues to several mushrooms including the published Tuber genome. So please see the links for  DNA extraction and extracting DNA from fruiting bodies.

If you are interested in contributing samples to the sequencing especially after making collections on a foray, please take note of the guidelines above and see the JGI 1000 Fungal Genomes list to identify where there are missing taxa which could be contributed. Simply fill out a nomination on the page for a species you can or have collected and it will be evaluated and if it passes the requirements, confirmed for nomination.

Grad Students & Postdocs, please also see how you can propose a species for sequencing through the Grad student / Postdoc challenge which will include these fruiting body samples.

Byssothecium circinans genome released

1KFG is pleased to announce the public release of the Byssothecium circinans  CBS 113818.  Bcircinans  is classified in the family Massarinaceae (Ascomycota; Dothideomycetes;Pleosporales), and is currently the only completed genomes from this family.

Byssothecium circinans  was contributed to the 1KFG project by Pedro Crous and Manfred Binder at CBS-KNAW.  As always, please contact the PIs associated with unpublished 1KFG genomes for permission prior to the use of any data in publications.

Coniochaeta sp genome release

1KFG is pleased to announce the public release of the genome of Coniochaeta sp. PMI_546, a fungus in the Coniochaetales family (Ascomyocta; Sordariomycetes) found living as saprotrophs, leaf and root endophytes, and plant pathogens. This isolate was cultivated from roots of the eastern cottonwood Populus deltoides.

This isolate was collected as part of the Plant Microbe Interface DOE project by Greg Bonito and Rytas Vilgalys. For use of these data prior to publication please contact these PIs.

Clavulina sp genome sequenced

1KFG is pleased to announce the public release of the genome of Clavulina sp. PMI_390 , a Basidiomycete fungus in the family Clavulinaceae (Cantharellales) that forms coral fungi structures. It is found as an ECM fungus associated with roots of pine and poplar trees.

This isolate was collected as part of the Plant Microbe Interface DOE project by Greg Bonito and Rytas Vilgalys. For use of these data prior to publication please contact these PIs.

Thozetella sp. PMI_491 genome released

1KFG is pleased to announce the public release of the genome of Thozetella sp. PMI_491 (Berkeley 1881), a Chaetosphaeriaceae fungus found growing on bark in Australia.

This isolate was collected as part of the Plant Microbe Interface DOE project by Greg Bonito and Rytas Vilgalys. For use of these data prior to publication please contact these PIs.

Thozetella spores

Sequencing unsampled fungal diversity