Protocols for fruiting body sampling

Recently 1KFG has begun exploring using fresh fruiting body sampled tissue to obtain DNA rather than requiring pure culture-based samples only. This can be problematic for a variety of reasons including the heterokaryon nature of fruiting tissue, possible contamination with bacteria, insects, or other fungi, and the limitations to return to the exact sample for additional sampling since it will either be destroyed in the sampling or only have a small amount left for a voucher specimen.

All of that noted, we still can only obtain samples of some fungi as fruiting mushrooms. The INRA-Nancy group have applied this approach of sampling fruiting tissues to several mushrooms including the published Tuber genome. So please see the links for  DNA extraction and extracting DNA from fruiting bodies.

If you are interested in contributing samples to the sequencing especially after making collections on a foray, please take note of the guidelines above and see the JGI 1000 Fungal Genomes list to identify where there are missing taxa which could be contributed. Simply fill out a nomination on the page for a species you can or have collected and it will be evaluated and if it passes the requirements, confirmed for nomination.

Grad Students & Postdocs, please also see how you can propose a species for sequencing through the Grad student / Postdoc challenge which will include these fruiting body samples.

Byssothecium circinans genome released

1KFG is pleased to announce the public release of the Byssothecium circinans  CBS 113818.  Bcircinans  is classified in the family Massarinaceae (Ascomycota; Dothideomycetes;Pleosporales), and is currently the only completed genomes from this family.

Byssothecium circinans  was contributed to the 1KFG project by Pedro Crous and Manfred Binder at CBS-KNAW.  As always, please contact the PIs associated with unpublished 1KFG genomes for permission prior to the use of any data in publications.

Coniochaeta sp genome release

1KFG is pleased to announce the public release of the genome of Coniochaeta sp. PMI_546, a fungus in the Coniochaetales family (Ascomyocta; Sordariomycetes) found living as saprotrophs, leaf and root endophytes, and plant pathogens. This isolate was cultivated from roots of the eastern cottonwood Populus deltoides.

This isolate was collected as part of the Plant Microbe Interface DOE project by Greg Bonito and Rytas Vilgalys. For use of these data prior to publication please contact these PIs.

Clavulina sp genome sequenced

1KFG is pleased to announce the public release of the genome of Clavulina sp. PMI_390 , a Basidiomycete fungus in the family Clavulinaceae (Cantharellales) that forms coral fungi structures. It is found as an ECM fungus associated with roots of pine and poplar trees.

This isolate was collected as part of the Plant Microbe Interface DOE project by Greg Bonito and Rytas Vilgalys. For use of these data prior to publication please contact these PIs.

Thozetella sp. PMI_491 genome released

1KFG is pleased to announce the public release of the genome of Thozetella sp. PMI_491 (Berkeley 1881), a Chaetosphaeriaceae fungus found growing on bark in Australia.

This isolate was collected as part of the Plant Microbe Interface DOE project by Greg Bonito and Rytas Vilgalys. For use of these data prior to publication please contact these PIs.

Thozetella spores

Corynespora cassiicola genome release

1KFG is pleased to announce the public release of the genome of Corynespora cassiicola (Berk.& M. A. Curtis), a plant pathogen of tropical and subtropical plants.

C. cassiicola

C. cassiicola forms an outgroup among the Pleosporales in the phylogenetic tree of the Dothideomycetes, without clear relationship to any other currently defined family. Corynespora cassiicola was contributed to the 1KFG project by Francis Martin (INRA) and Valérie Pujade-Renaud (CIRAD).

Ophiobolus disseminans genome release

1KFG is pleased to announce the public release of the Ophiobolus disseminans CBS 113818O. disseminans  is classified in the family Leptosphaeriaceae (Ascomycota; Dothideomycetes;Pleosporales), and is one of two completed genomes from this family.

Ophiobolus disseminans was contributed to the 1KFG project by Pedro Crous and Manfred Binder at CBS-KNAW.  As always, please contact the PIs associated with unpublished 1KFG genomes for permission prior to the use of any data in publications.

Lophium mytilinum genome release

1KFG is pleased to announce the public release of the Lophium mytilinum.  L. mytilinum  is classified in the family Mytilinidiaceae (Ascomycota; Dothideomycetes;Mytilinidiale), and is one of four proposed sequenced genome from this family.

Lophium mytilinum was contributed to the 1KFG project by Pedro Crous and Manfred Binder at CBS-KNAW.  As always, please contact the PIs associated with unpublished 1KFG genomes for permission prior to the use of any data in publications.

Aaosphaeria arxii genome release

1KFG is pleased to announce the public release of the Aaosphaeria arxii (Aa) Aptroot.  A. arxii  is classified in the family Dacampiaceae (Ascomycota; Dothideomycetes; Pleosporales), and is one of two sequenced genome from this family. This isolate was found growing on corn plants in Columbia and has been isolated from other plants.

Aaosphaeria arxii was contributed to the 1KFG project by Pedro Crous and Manfred Binder at CBS-KNAW.  As always, please contact the PIs associated with unpublished 1KFG genomes for permission prior to the use of any data in publications.

Macroventuria anomochaeta genome release

1KFG is pleased to announce the public release of the Macroventuria anomochaeta van der Aa genome.   M. anomochaeta is classified in the family Didymellaceae (Ascomycota; Dothideomycetes; Pleosporales), and is one of two sequenced genome from this family. This isolate was found on decaying canvas in the desert in South Africa.

Macroventuria anomochaeta was contributed to the 1KFG project by Pedro Crous and Manfred Binder at CBS-KNAW.  As always, please contact the PIs associated with unpublished 1KFG genomes for permission prior to the use of any data in publications.

Sequencing unsampled fungal diversity